other:Article Title: Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti β-ACTIN Sigma Cat# A2228-100UL RRID: AB_476697 Mouse anti BAG1 Santa Cruz Biotech Cat# sc-33704 RRID: AB_626720 Rabbit anti BAG3 Abcam Cat# ab47124, RRID: AB_867788 Mouse anti Cas9 Cell Signaling Tech Cat# 14697S, RRID: AB_2750916 Mouse anti Ki67 Dako Cat# M7240, RRID: AB_2142367 Mouse anti GAPDH-HRP Santa Cruz Biotech Cat# sc-47724 RRID: AB_627678 Rabbit anti LMNB1 Abcam Cat# ab16048, RRID: AB_443298 Rabbit anti NAE1 Cell Signaling Tech Cat# 14321, RRID: AB_2798448 Rabbit anti NEDD8 Abcam Cat# ab81264, RRID: AB_1640720 Rabbit anti p21 Cell Signaling Tech Cat# 2947, RRID: AB_823586 Mouse anti TAU Thermo Fisher Cat# MN1000 RRID: AB_2314654 Mouse anti p-TAU(S202/T205) Thermo Fisher Cat# MN1020, RRID: AB_223647 Rabbit anti UBA3 Abcam Cat# ab124728, RRID: AB_10974248 Mouse anti phospho-ATM(S1981) Thermo Fisher Cat# MA1-2020, RRID: AB_1086244 Rabbit anti Cleaved Caspase-3 (Asp175) Cell Signaling Tech Cat# 9661, RRID: AB_2341188) Mouse anti p53 Cell Signaling Tech (Cat# 48818, RRID: AB_2713958) Rabbit anti FOXG1 TaKaRa Cat# M227 RRID: AB_2827749 Chicken anti MAP2 Thermo Fisher Cat# PA1-16751 RRID: AB_2138189 Rabbit anti NANOG Thermo Fisher Cat# 4903, RRID: AB_10559205 Rabbit anti PAX6 Biolegend Cat# 901301 RRID: AB_2749901 Mouse anti SENP8 Santa Cruz Biotech Cat# sc-271498 RRID: AB_10649498 Mouse anti p62 Lck Ligand BD Bioscience Cat# 610832 RRID: AB_398151 Rabbit anti LC3A/B XP Cell Signaling Tech Cat# 12741S RRID: AB_2617131 Rabbit p-TAU(S235) Thermo Fisher Cat# PA5-104785, RRID: AB_2816258 (Continued on next page) Cell Stem Cell 33, 1–5, February 5, 2026 © 2025 The Author(s).
Article Title: Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-rabbit IgG H&L (Alexa Fluor 555) Invitrogen Cat# A27039; RRID:AB_2536100 Goat anti-chicken IgG H&L (Alexa Fluor 647) Invitrogen Cat# A21449; RRID:AB_2535866 Bacterial and virus strains One Shot Stbl3 Chemically Competent E. coli Thermo Fisher C737303 Chemicals, peptides, and recombinant proteins Halt Protease Inhibitor Thermo Fisher 87786 Halt Phosphatase inhibitor Thermo Fisher 78420 Y-27632 R&D Systems 1254 SB431542 R&D Systems 1614 LDN193189 (LDN) Stemgent 04-0074 CHIR99021 R&D Systems 4432 SHH C25II R&D Systems 464-SH Poly-L-Ornithine (PO) Sigma Aldrich P3655 Mouse Laminin I (LAM) R&D Systems 3400-010-1 Fibronectin (FN) Thermo Fisher Scientific 356008 0.5M EDTA, pH 8.0 Thermo Fisher Scientific 15575020 Accutase Innovative Cell Technologies AT104-500 Trypsin-EDTA 0.05% Thermo Fisher Scientific 25300-054 GDNF Peprotech 450-10 AA Sigma 4034-100g cAMP Sigma D0627 BDNF R&D Systems 248-BD TGFb3 R&D Systems 243-B3 DAPT R&D Systems 2634 DMEM:F12 w/ high glucose Thermo Fisher Scientific 11320-033 E8 medium Thermo Fisher A1517001 E6 medium Thermo Fisher A1516401 DMEM/F12 Thermo Fisher 21331020 Neurobasal medium Life Technologies 21103-049 100X N2 supplement Life Technologies 17502-048 50X B27 supplement w/o Vit A Life Technologies 12587-010 L-Glutamine (100X) Thermo Fisher Scientific 25030-081 Penicillin Streptomycin Thermo Fisher Scientific 15140-122 4’,6-diamidino-2-phenylindole (DAPI) Sigma D9542 Matrigel BD Biosciences 356234 Vitronectin (VTN-N) Thermo Fisher Scientific A14700 Geltrex Life Technologies A1413201 Trizol Thermo Fisher Scientific 15596026 STEM-CELLBANKER Amsbio 11890 4% paraformaldehyde Affymetrix MFCD00133991 Neuron Isolation Enzyme Thermo Fisher Scientific 88285 Precision Red Advanced Protein Assay Cytoskeleton, Inc ADV02-A DPBS Thermo Fisher Scientific 14190-136 CellEvent Senescence Green Thermo Fisher Scientific C10840 X-tremeGENE HP Sigma 6366236001 Doxycycline hyclate Sigma D9891 Proteostat Enzo Life Sciences ENZ-51023-KP050 MLN4924 (Pevonedistat) MedChemExpress HY-70062 CSN5i3 Tocris 7089 (Continued on next page) e2 Cell Stem Cell 31, 1162–1174.e1–e8, August 1, 2024
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